Systems for proteomics

Discover Enzymatic Digestion


Discover Enzymatic Digestion



Ideal for: membrane solubilizations - dramatically increases protein recovery, ICAT & iTRAQTM tagging - improves tagging efficiency, In-gel & Solution digests - 5-10 minute digests with improved coverage

We help chemists explore the unlimited potential of their creativity. The Discover® System is the most versatile and convenient platform available for microwave-assisted organic synthesis. CEM offers an array of modules for the base system that can be used with "plug and play" ease, making Discover both customizable and extremely cost-effective.



Product Advantages



  • FocusedTM Microwave cavity design for the most efficient use of energy

  • Precise control of temperature and power

  • Simultaneous cooling - patented feature that allows microwave energy input while maintaining low to moderate temperatures

  • Flexible - open architecture design allows introduction of a variety of different sample types



 





Discover Protein Hydrolysis


Discover Protein Hydrolysis



Reliable preparation of protein hydrolysates is the rate determining step for accuracy and precision in amino acid analysis. The widely used conventional protocol developed by Stein and Moore in the 1950's involves heating samples in 6N HCL for periods of 24 hours or more.

Advances in HPLC instrumentation allow accurate analysis on minute amounts (<50 picomoles) of sample in less than one hour. Unfortunately, the improved sensitivity and separation times of amino acid analyzers are offset by the contamination, run to run variability, and time associated with conventional hydrolysis procedures.

Microwave hydrolysis represents an accurate and convenient alternative to conventional hydrolysis techniques. Protein hydrolysates can be prepared in less time than a single chromatographic run, eliminating this rate-limiting step or "bottleneck" in amino acid analysis without compromising accuracy and precision.

Microwave hydrolysis accelerates the rate of reaction without altering the fundamental chemistry of amino acid analysis. The same acids, protective agents and derivitization chemistries can be utilized in microwave hydrolysis techniques. It reduces the time required for cleavage of difficult to hydrolyze hydrophobic peptide linkages without excessive degradation of the labile amino acids: serine and threonine.



 


News

A legend is born

The all new ARTUS 8 is the latest addition to ARUN’s portfolio of metal analysers. It completes the offering to higher end applications. Its primary field of applications are in foundries, metal producing and metal processing industries.
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Do not miss out! New campaign from RETSCH GmbH called "The Future is Now - New Dimensions in Sample Preparation and Particle Analysis".
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CEM brings a remarkable intelligence and ease of use to microwave sample preparation with the new MARS 6 System!
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